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We recently identified a novel phospholipase Cdelta isoform, PLC-deltasu, in sea urchin gametes, whose precise functional role during fertilization and early embryogenesis remains unknown. Here, we characterized the binding of the PLC-deltasu PH domain to different phosphatidylinositol (PI) phospholipids and studied changes in its localization during fertilization. The PLC-deltasu PH domain bound most strongly to PI(3,4)P(2) and PI(3,5)P(2) phospholipids, in contrast to the PLCdelta1 PH domain which bound predominantly to PI(4,5)P(2). A green fluorescent protein tagged PLC-deltasu PH domain localized to the plasma membrane and its localization increased at fertilization and following addition of a Ca(2+) ionophore. However, recombinant PLC-deltasu failed to cause Ca(2+) signals like those seen at fertilization, in mouse and sea urchin eggs. Our findings suggest that PLC-deltasu is unlikely to be directly involved in the process of egg activation but may play a role in mediating extracellular signals transmitted via the PI 3'-kinase pathway.

Original publication

DOI

10.1016/j.bbrc.2007.04.050

Type

Journal article

Journal

Biochem Biophys Res Commun

Publication Date

15/06/2007

Volume

357

Pages

964 - 970

Keywords

Animals, Cells, Cultured, Embryonic Development, Fertilization, Isoenzymes, Mice, Phospholipase C delta, Phospholipids, Protein Binding, Sea Urchins, Type C Phospholipases, Zygote