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Expression microarrays have great potential for clinical use but variability of the results represents a challenge for reliable practical application. The amount of fluorescent dye used in microarray experiments is a significant source of variability that has not been systematically studied. Here we demonstrate that the quantity of Cy3 dye affects microarray results performed on tumor specimens. Signal-to-noise ratios and coefficients of variation are significantly improved by increasing Cy3 to 150-180 pmol, but any further increase does not improve the data. In conclusion, optimal amounts of dye reduce variability and improve reliability of expression microarray experiments.

Original publication

DOI

10.1385/MB:30:2:151

Type

Journal article

Journal

Mol Biotechnol

Publication Date

06/2005

Volume

30

Pages

151 - 154

Keywords

Carbocyanines, Fluorescent Dyes, Gene Expression Profiling, Humans, Neoplasms, Oligonucleotide Array Sequence Analysis