Extracellular vesicle sizing and enumeration by nanoparticle tracking analysis.
Gardiner C., Ferreira YJ., Dragovic RA., Redman CW., Sargent IL.
Nanoparticle tracking analysis (NTA) is a light-scattering technique that is useful for the rapid sizing and enumeration of extracellular vesicles (EVs). As a relatively new method, NTA has been criticised for a lack of standardisation. We propose the use of silica microspheres for the calibration of NTA measurements and describe in detail a protocol for the analysis of EVs by NTA which should minimise many of the sources of variability and imprecision associated with this technique.