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Nanoparticle tracking analysis (NTA) is a light-scattering technique that is useful for the rapid sizing and enumeration of extracellular vesicles (EVs). As a relatively new method, NTA has been criticised for a lack of standardisation. We propose the use of silica microspheres for the calibration of NTA measurements and describe in detail a protocol for the analysis of EVs by NTA which should minimise many of the sources of variability and imprecision associated with this technique.

Original publication

DOI

10.3402/jev.v2i0.19671

Type

Journal article

Journal

J Extracell Vesicles

Publication Date

2013

Volume

2

Keywords

extracellular vesicles, light scattering, nanoparticle tracking analysis, standardisation