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Highly quantitative histological analysis was used to validate the technique of direct intra-ovarian biopsy in anaesthetised broodstock tilapia, Tilapia zillii (Gervais), as a simple and effective means of assessing ovarian maturity in vivo without unnecessary sacrifice. Stereology, a proven mathematical method for the highly quantitative analysis of histomorphometry, demonstrated that small pieces of ovarian tissue collected by intra-ovarian cannulation did not differ significantly (P≥ 0.05) from the remaining ovary in terms of the relative proportion of any oocyte developmental stage. © 2001 Elsevier Science B.V.

Original publication

DOI

10.1016/S0044-8486(00)00544-5

Type

Journal article

Journal

Aquaculture

Publication Date

02/04/2001

Volume

195

Pages

183 - 188