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Methods have been developed for isolating human tissue macrophages from first trimester or term pregnancy decidua. After a two stage enzymic digestion, viable cells were separated from cellular debris by velocity sedimentation at unit gravity or by Percoll centrifugation. Cell populations were analysed by flow cytometry after labelling with monoclonal antibodies. In term decidua, 47% of the cells were of bone marrow origin, comprising 18% macrophages, 3% large granular lymphocytes and 8% T cells. The remaining cells, the proportion of which varied between individuals, were CD16-positive granulocytes. Macrophages were isolated flow cytometrically from both first trimester and term decidual cell dispersions after labelling with an antibody to MHC class II. Yields of up to 4 X 10(6) macrophages, greater than 95% pure, were routinely obtained.

Type

Journal article

Journal

J Immunol Methods

Publication Date

14/09/1990

Volume

132

Pages

181 - 189

Keywords

Antibodies, Monoclonal, Antigens, CD, Antigens, CD3, Antigens, Differentiation, T-Lymphocyte, Cell Separation, Centrifugation, Density Gradient, Decidua, Female, Flow Cytometry, HLA Antigens, Humans, Macrophages, Pregnancy, Pregnancy Trimester, First, Pregnancy Trimester, Third, Receptors, Antigen, T-Cell