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Term cytotrophoblast do not express polymorphic MHC Class I antigens, unlike other fetal and maternal cells in the amniochorion/decidua. This allows cytotrophoblast to be isolated and purified from this tissue, utilizing 4E, a monoclonal antibody specific for HLA-B, which labels only non-trophoblast. We have developed a method using enzymic dispersion and Percoll gradient centrifugation, followed by flow cytometry, that yields, on average, a total of 5 X 10(6) term extravillous cytotrophoblast, 97 per cent pure. The availability of highly purified extravillous cytotrophoblast, for the first time, permits precise investigation of trophoblast function.

Type

Journal article

Journal

Placenta

Publication Date

11/1990

Volume

11

Pages

505 - 513

Keywords

Antibodies, Monoclonal, Cell Separation, Centrifugation, Density Gradient, Female, Flow Cytometry, Humans, Immunohistochemistry, In Vitro Techniques, Placenta, Trophoblasts