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The large and complex genome of wheat makes genetic and genomic analysis in this important species both expensive and resource intensive. The application of next-generation sequencing technologies is particularly resource intensive, with at least 17 Gbp of sequence data required to obtain minimal (1×) coverage of the genome. A similar volume of data would represent almost 40× coverage of the rice genome. Progress can be made through the establishment of consortia to produce shared genomic resources. Australian wheat genome researchers, working with Bioplatforms Australia, have collaborated in a national initiative to establish a genetic diversity dataset representing Australian wheat germplasm based on whole genome next-generation sequencing data. Here, we describe the establishment and validation of this resource which can provide a model for broader international initiatives for the analysis of large and complex genomes.

Original publication

DOI

10.1111/j.1467-7652.2012.00717.x

Type

Journal article

Journal

Plant Biotechnol J

Publication Date

08/2012

Volume

10

Pages

703 - 708

Keywords

Australia, Databases, Genetic, Genetic Variation, Genome, Plant, Polymorphism, Single Nucleotide, Sequence Analysis, DNA, Triticum