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A substantial number of GH regulated genes have been reported in mature hepatocytes, but genes involved in GH-initiated cell differentiation have not yet been identified. Here we have studied a well-characterised model of GH-dependent differentiation, adipogenesis of 3T3-F442A preadipocytes, to identify genes rapidly induced by GH. Using the suppression subtractive hybridisation technique, we have identified eight genes induced within 60 min of GH treatment, and verified these by northern analysis. Six were identifiable as Stat 2, Stat 3, thrombospondin-1, oncostatin M receptor beta chain, a DEAD box RNA helicase, and muscleblind, a developmental transcription factor. Bioinformatic approaches assigned one of the two remaining unknown genes as a novel 436 residue serine/threonine kinase. As each of the identified genes have important developmental roles, they may be important in initiating GH-induced adipogenesis.

Type

Journal article

Journal

Mol Cell Endocrinol

Publication Date

28/03/2002

Volume

189

Pages

213 - 219

Keywords

Adipocytes, Amino Acid Sequence, Cell Differentiation, Cell Line, DNA-Binding Proteins, Drosophila Proteins, Gene Expression Regulation, Gene Library, Growth Hormone, Humans, Molecular Sequence Data, Nuclear Proteins, Protein-Serine-Threonine Kinases, RNA Helicases, Receptors, Cytokine, Receptors, Oncostatin M, STAT2 Transcription Factor, STAT3 Transcription Factor, Thrombospondin 1, Trans-Activators